ISOSTRIP MOUSE MAB ISOTYPING KIT

General description

IsoStrip Mouse Monoclonal Antibody Isotyping Kit is a simple, three-step kit for the rapid characterization of mouse monoclonal antibodies.

Contents

• Development Tubes, contain lyophilized latex beads, precoated with anti-mouse-Ig antibodies
• Isotyping Strips, precoated with subclass- and light-chain-specific anti-mouse-Ig antibodies

Specificity

Anti-mouse antibodies, bound to the latex beads, will react with any mouse monoclonal antibody regardless of its isotype.Goat anti-mouse antibodies, immobilized to the strip, specifically bind to each of the common mouse antibody isotypes (IgG₁, IgG_2a, IgG_2b, IgG₃, IgM, and IgA), and to the light chains.

Specificity for all specific antibodies beside IgML at 1.0 μg/ml: ≥ 2
IgM Lambda, at 1.0 μg/ml: ≥ 1

Application

The kit is used for the rapid identification of class, subclass, and light-chain types of mouse monoclonal antibodies (immunoglobulins).

Features and Benefits

• Reliable: Yields comparable results to the standard ELISA assay
• Fast: 5 minutes from start to finish
• Easy: Only 3 steps
• Economical: No equipment or additional reagents required
• Highly specific: No crossreactivity with FBS

Packaging

1 kit containing 2 components.

Specifications

Assay time: 5 minutes
Sample material: Hybridoma supernatant, purified mouse monoclonal antibodies, ascites

Preparation Note

Working solution: Dilute Sample

• Remove the desired number of isotyping strips from the canister. Remove the caps from an equal number of development tubes.
  The tubes may be labeled with a pencil or felt-tipped lab marker for easy identification.
• Dilute a sample containing the mouse monoclonal antibody in 1% BSA/ phosphate-buffered saline (PBS), pH 7.2 to 7.6.
  Culture supernatant samples should be diluted 1:10 to 1:100. Ascites samples should be diluted 1:20,000. These are recommended dilutions and may vary depending on the concentration of antibody in your sample. In our experience, a monoclonal antibody
  concentration of 0.1 to 1 g/ml of diluted sample gives the best results. 150 ml of this diluted sample will be added to the development tube.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

IsoStrip is a trademark of Roche