iTRAQ(R) Reagent - 8PLEX Multiplex Kit (C15-1303-569)

General description

Contains iTRAQ^® Reagents 113, 114, 115, 116, 117, 118, 119 and 121 and sufficient material for five 8-plex assays. Multiplex Buffer kit (Cat. No. 4381664) to be ordered separately.

Cat. No. 4381663 contains the equivalent reagents in the Sciex iChemistry^® product number 4390812.

The isobaric tags for relative and absolute quantitation (iTRAQ^®) reagents are the first set of multiplexed, amine-specific, stable-isotope reagents that can label all peptides in up to eight different biological samples enabling simultaneous identification and quantitation, relative and absolute, while retaining important post-translational modification (PTM) information. The iTRAQ Reagents - 8plex kits are available in more economical bulk packs.

Application

iTRAQ^® Reagent - 8PLEX Multiplex Kit has been used:

• to derivatize and label the tryptic digests of the iodoacetamide probe (IPM)-labeled HeLa proteome
• in isobaric tags for relative and absolute quantitation (iTRAQ) labeling of peptides
• as isobaric labels for the comparative quantification of proteins between the digested samples in proteomic analysis

Features and Benefits

• Simultaneously identify and quantify proteins from multiple samples
• Expands protein and proteome coverage by labeling all peptides, including those with post-translational modifications (PTMs)
• Increases confidence in identification and quantitation from MS/MS spectra by tagging multiple peptides per protein
• Increases throughput and confidence in results for protein biomarker discovery studies
• Offers a simple workflow without sample fractionation for reduced-complexity samples, such as affinity pull-downs
• Provides the flexibility to multiplex up to eight different biological samples simultaneously in a single experiment
• Fully supported by ProteinPilot Software on all SCIEX proteomics LC/MS/MS platforms

Analysis Note

To view the Protocol for the iTRAQ Reagents 8-plex Kit, please visit this Protocol link.

To view the Chemistry Quick Reference Card for the iTRAQ Reagents, please visit this Chemistry Quick Reference Card link.

Legal Information

iChemistry is a registered trademark of AB Sciex Pte. Ltd.

iTraq is a registered trademark of AB Sciex Pte. Ltd.

Quality Level: 100. storage temp.: −. 20°C. Pictograms: GHS02,GHS07. Signal Word: Danger. Hazard Statements: H225 - H302 + H312 + H332 - H319. Precautionary Statements: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338. Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2. Storage Class Code: 3 - Flammable liquids. WGK: WGK 3. Flash Point(F): 35.6 °F - closed cup. Flash Point(C): 2.0 °C - closed cup.