J774A.1 MOUSE BALB/C MONOCYTE MACROPHAG (C15-1312-375)

Cell Line Origin

Mouse BALB/c monocyte macrophage

Cell Line Description

Derived from a tumour in a female BALB/c mouse. Cell line produces IL-5 and lysozyme.

Application

J774A.1 cell line has been used to test the cytotoxicity of [1,2,3]triazolo[1,5-a]pyridinium salts (having leishmanicidal activity) from triazolopyridines. It has also been used to determine the antitrypanosomatid activity of flavonoid glycosides isolated from Delphinium sps.

Metabolic studies

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Cells are subcultured by harvesting attached and suspension cells. Remove majority of medium and centrifuge to harvest cells growing in suspension. Scrape adherent cells remaining in the flask. Resuspend cell pellet from centrifugation and combine with scraped cells. Seed into new flask at 1:3 to 1:4 split ratio i.e. seeding at 1-3 x 10,000 cells/cm² Incubate at 37°C; 5% CO₂. Please Note :- On receipt growing order cells may be in suspension. The cells and media should be removed from the flask and spun down to form a pellet. This should then be re-suspended in fresh media and a cell count performed. The cells should then be reseeded at 2-4 x 10E4/cm and should begin to show signs of re-attachment within 48 hours.

Other Notes

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biological source: mouse blood. description: Mouse BALB/c monocyte macrophage. growth mode: Semi-adherent. karyotype: Not specified. morphology: Not specified. products: Interleukin 1 (IL-1), lysozyme. receptors: Immunoglobulin, complement. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.