JC-10

JC-10 is an ideal fluorescent probe widely used to detect mitochondrial membrane potential △Ψm. Can detect cell, tissue or purified mitochondrial membrane potential. When the mitochondrial membrane potential is high, JC-10 aggregates in the matrix of the mitochondria to form a polymer, which can produce red fluorescence; when the mitochondrial membrane potential is low, JC-10 cannot accumulate in the matrix of the mitochondria, at this time JC- 10 is a monomer, which can produce green fluorescence. In this way, it is very convenient to detect the change of mitochondrial membrane potential through the change of fluorescence color. The relative ratio of red and green fluorescence is commonly used to measure the ratio of mitochondrial depolarization. The decrease of mitochondrial membrane potential is a landmark event in the early stage of apoptosis. The decrease of cell membrane potential can be easily detected by the transition of JC-10 from red fluorescence to green fluorescence, and the transition from red fluorescence to green fluorescence of JC-10 can also be used as an early detection indicator of cell apoptosis. The maximum excitation wavelength of JC-10 monomer is 515nm and the maximum emission wavelength is 529nm; the maximum excitation wavelength of JC-10 polymer is 585nm and the maximum emission wavelength is 590nm. For actual observation, use the conventional settings for observing red fluorescence and green fluorescence. Specification: 0.95 Molecular Formula: C25H29Cl2IN4 Molecular Weight: 583.34 Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/J141206-SCI_6d382918d8e0df77744420e9bd678caa.pdf