Cell Line Origin
Rat sarcoma, asparagine requiring
Cell Line Description
Used in studies relating to amino acid requirements and L-asparagine metabolism. Cloning studies show that variants not requiring L-asp are present in 100 per 1,000,000 cells Cloned populations which do not produce variants immediately do so after 6-8 further passages.
Application
Metabolic and cloning studies
Culture Medium
McCoy′s 5a + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Subculture Routine
Renew medium daily where possible. Subculture by placing culture in cold room (4°C) for 60 minutes, shake flask to suspend cells, centrifuge, resuspend in fresh medium and into fresh flasks. Split sub-confluent cultures (70-80%) 1:2 to 1:10; 5% CO2; 37°C.
Other Notes
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biological source: rat unknown/unspecified. growth mode: Adherent. karyotype: 2n : 42, hyperdiploid. morphology: Fibroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51112522
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 90111913-1VL
