General description
JumpStart™ Taq ReadyMix™ For High Throughput Quantitative PCR combines the performance enhancements of JumpStart Taq Antibody for hot start PCR with the convenience of an easy-to-use reaction mixture that incorporates the internal reference dye for ABI and other real time instrument applications. This 2× concentrate master mix contains JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer. Simply add an equal volume of the 2× ReadyMix to a 2× mixture DNA template, primers and fluorescent probe.
To prepare a typical qPCR reaction, mix 25 μL of JumpStart ReadyMix Taq, fluorescent probe, desired amount of magnesium chloride (above 1.5 mM final concentration), template DNA, and primers in a final volume of 50 μL. Reaction volumes can be scaled down, if desired.
Application
JumpStart™ Taq ReadyMix™ for High Throughput Quantitative PCR has been used:
- For quantitative polymerase chain reaction (qPCR) of cDNA template
- As a component of PCR reaction mix for amplification of DNA extracted from peripheral blood leucocytes
- For qPCR amplifications using SYBR
- For multiplex PCR
- For reduction of primer dimers
Features and Benefits
- JumpStart™ Taq DNA polymerase prevents amplification of non-specific products, resulting in increased efficiency and higher target yield
- When performing large number of PCR reactions, JumpStart™ Taq ReadyMix™ reduces preparation time and the risk of contamination from multiple pipetting steps
- This master mix allows consistent batch-to-batch and reaction-to-reaction performance
- Internal Reference Dye is provided for reaction normalization
Packaging
Default reaction volume is 50 μL
20RXN is packaged as 1 X 500 μL
400RXN is packaged as 1 X 10 mL
2000RXN is packaged as 1 X 50 mL
Quantity
Use 2.5 units per reaction.
Legal Information
Eppendorf is a registered trademark of Eppendorf AG
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
- UPC:
- 41106300
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- D6442-400RXN
