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JURKAT E6.1HUMAN LEUKAEMIC T CELL LYMPHO RNA

Sigma Aldrich

Catalog No.
C15-1632-990
Manufacturer No.
88042803-RNA-5UG
Manufacturer Name
Sigma-Aldrich
Quantity
5
Unit of Measure
EA
Price: $881.29
List Price: $979.21

Cell Line Origin Human leukaemic T cell lymphoblast Cell Line Description Derived from Jurkat FHCRC. An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages.

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Cell Line Origin

Human leukaemic T cell lymphoblast

Cell Line Description

Derived from Jurkat FHCRC. An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages.

Application

IL-2 assay and cloning studies

Jurkat E6.1 cell line has been used to:

  • study cell-independent/vector-free delivery of bovine serum albumin-fluorescein conjugate
  • determine the cytotoxicity of the cytolethal distending toxin (CDT) holotoxin and its components
  • study the expression of bisphenol A exposed estrogen receptor-β (ERβ) and estrogen-related-receptor-α (ERRα) in vitro

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. On resuscitation single cells can be observed, during culture most cells will grow as aggregates.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

UPC:
41116127
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
WeightUOM:
LB
MPN:
88042803-RNA-5UG
Product Size:
RNA-5UG

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