Cell Line Origin
Human thyroid carcinoma
Cell Line Description
Derived from a primary papillary thyroid carcinoma. Retains thyroid follicular cell differentiation e.g. thyroglobulin synthesis. Expresses wild-type p53 tumour suppresser gene. It has been reported that K1 cells have their origin in the thyroid papillary carcinoma cell line GLAG-66.
Application
K1 Cell Line human has also been used to study and correlate the expression levels of c-KIT (a receptor tyrosine kinase) and two markers of thyrocyte differentiation (PAX8 and TTF-1).
Tumourigenicity studies, particularly the role of p53
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11,12
D5S818: 10,11
D7S820: 11
THO1: 6,9
TPOX: 8
vWA: 17,18
Culture Medium
DMEM:Ham′s F12:MCDB 105 (2:1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2x1,000,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: human thyroid. description: Human thyroid carcinoma. growth mode: Adherent. karyotype: Not specified. morphology: Not specified. products: Wild-type p53 tumour suppressor gene. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51111643
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 92030501-1VL
