Kininogen high molecular weight, Two Chain from human plasma

General description

HK (KNG1) gene is mapped to human chromosome 3q27.3. It is a glycoprotein.

High-molecular-weight kininogen (HK) is the substrate and cofactor of the plasma kallikrein-kinin system (KKS). It is present at a concentration of 660 nM on the plasma and binds to platelet GPI-V-IX complex.

Biochem/physiol Actions

High-molecular-weight kininogen (HK) inhibits binding of thrombin to platelets.

High molecular weight kininogen, two chain, is a participant in contact phase activation of the intrinsic blood coagulation cascade that forms a substrate on which blood factor XI is held in proximity to factor XII allowing reciprocal activation of the two factors. It is also involved in the activation of prekallikrein. High molecular weight kininogen binds to proteoglycans on the surface of platelets, endothelial cells, and other cell types. Domain 5 of high molecular weight kininogen inhibits platelet aggregation and angiogenesis.

Packaging

Package size based on protein content

Physical form

Lyophilized from 4 mM sodium acetate, pH 5.3, 0.15 M NaCl

Preparation Note

Prepared by kallikrein digestion of human kininogen. Purified to remove kallikrein and kinin.