Cell Line Origin
Rat Lou/MN spleen x Mouse Sp2/0
Cell Line Description
The hybridoma KM703 was produced by the fusion of Sp2/0 mouse myeloma cells with spleen cells from a Lou/MN rat immunised with the bone marrow derived stromal cell clone, BMS2. The monoclonal antibody recognises an epitope on Pgp-1, the murine homologue of CD44. It prevents the growth of lymphoid or myeloid cells in long term bone marrow cultures.
Immunogen
BUS 2 cells
Application
Determination of ligand specifities and function of Pgp-1/CD44 molecules.
The isotype of the monoclonal antibody produced by this cell line is rat IgG2a.
Culture Medium
RPMI 1640 + 2mM glutamine + 0.05mM 2-mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C
Other Notes
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biological source: Rat x Mouse Hybridoma. ImmunoDon: Rat Lou/MN spleen cells. Myeloma: Sp2/0. Reactivity: Anti-Pgp-1, murine homologue of CD44. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51342710
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 94090613-1VL
