Cell Line Origin
Human oesophageal squamous cell carcinoma
Cell Line Description
Established from the well differentiated invasive oesophageal squamous cell carcinoma resected from the middle intra-thoracic oesophagus of a 79-year-old Japanese man prior to treatment. This cell line has been mycoplasma eradicated at ECACC.
Culture Medium
RPMI 1640:HAMS F12 (1:1) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS)
Subculture Routine
Split confluent cultures 1:5 every second day using trypsin/EDTA;5% CO2; 37°C. Doubling time: 24 hours. Recommended seeding density: 2.5 x 10,000 cells/cm2.
Other Notes
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biological source: human esophagus. growth mode: Adherent. karyotype: Hypotriploid with 16% polyploidy. morphology: Epithelial. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51442717
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 94072021-1VL