Cell Line Origin
Rat lung
Cell Line Description
Derived from normal rat lung tissue. The cells possess elevated levels of specific enzymes involved in the synthesis of phosphatidyl choline. Osmiophilic lamellar inclusions are observed in cell pellets from mass culture.
Culture Medium
Coon′s modified Ham′s F12 or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: rat lung. growth mode: Adherent. karyotype: Modal no. 77. morphology: Epithelial. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51161721
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 90112811-1VL