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l-lactate assay kit (c15-1884-239)

L-LACTATE ASSAY KIT (C005B-448002)

Catalog No.
C005B-448002
Mfr. No.
MAK443-1KT
Mfr. Name
Sigma-Aldrich
Qty/UOM
1
UOM
KT
Price: $950.52
List Price: $1,056.13

General description Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when

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General description

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology.

Features and Benefits

  • Detection limit of 1 μM and linearity up to 50 μM L-lactate in 96-well plate assay.
  • Convenient room temperature assay. The procedure involves adding a single working reagent, and reading the fluorescence after 60 minutes.
  • Compatible with high-throughput handling systems.

Suitability

The kit is suitable for the quantitative determination of L-lactate (L-lactic acid) and the evaluation of drug effects on its metabolism in serum, plasma, urine, cell culture media, and other biological samples.

Principle

The L-Lactate Assay Kit is based on the lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λEx = 530 nm/λEm = 585 nm, is proportional to the lactate concentration in the sample.

Shipping Information:

Dry Ice Surcharge & Ice Pack Shipments: $40

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UPC:
41141620
Condition:
New
Availability:
3-5 Days
Weight:
1.00 Ounces
HazmatClass:
No
MPN:
MAK443-1KT
Temperature Control Device:
Yes

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