General description
Lactic Dehydrogenase (LDH) has a total molecular weight of 140 kDa and is composed of 4 subunits which are designated M subunit (muscle) and H subunit (heart). These subunits may be mixed in any of 5 combinations (M4, M3H1, M2H2, MH3, and H4). Skeletal muscle contains LDH that is predominately M4 with some small amounts of M3H and traces of H2H2. The H and M subunits are quite similar in molecular weight, but differ substantially in amino acid composition. Rabbit muscle LDH dissociates into dimeric species (MW = ~70 kDa) in acetate-chloride at pH 5.0, the dissociation is reversible. Biochemistry, 13, 3527-3531 (1974). Oxidizes glyoxylate and lactate.
Isoelectric point: 8.4-8.6
Optimal pH : 7.5 .
Application
- for protein-binding measurement
- to detect the action of oxaloacetate decarboxylase
- to determine serum
L - andD -lactate
Biochem/physiol Actions
Lactic dehydrogenase is responsible for the conversion of pyruvate to lactate in the fermentative metabolism.
Also catalyzes the oxidation of other
Unit Definition
One unit will reduce 1.0 μmole of pyruvate to
Physical form
Crystalline suspension in 3.2 M (NH4)2SO4 solution, pH 6.0
Analysis Note
Protein determined by biuret.
- UPC:
- 51391617
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- L2500-100KU