L48H37 (C09-1142-452)

L48H37 is an analog of Curcumin ( HY-N0005 ) with improved chemical stability. L48H37 is a potent and specific myeloid differentiation protein 2 (MD2) inhibitor and inhibits the interaction and signaling transduction of LPS-TLR4/MD2. L48H37 is used for the research of sepsis or lung injury treatmentIn VitroL48H37 inhibits LPS-induced inflammation, particularly TNF-α and IL-6 production and gene expression in mouse macrophages. L48H37 (0-20 µM; 24 hours) decreases the viability of A549 and H460 cells with IC 50 values of 5.3 µM and 2.3 µM, respectively, which is more effective compared to curcumin in lung cancer cells. It shows a low cytotoxicity on normal human lung epithelial cells (BEAS-2B) with IC 50 of 21 μM. L48H37 (1, 2, or 4 µM; 16 hours) dose‐dependently inhibited the expression of p‐Cdc2 and Cdc2, and increases the expression of p53. It also shows increased levels of cleaved poly (ADP‐ribosyl) polymerase (PARP) and reduced levels of anti‐apoptotic protein Bcl‐2 in H460 and A549 cells. L48H37 (4 µM; 16 hours) rapidly induces intracellular ROS levels dose-dependently as detected by increased DCF levels in H460 and A549 cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: A549 and H460 cells; BEAS-2B cells Concentration: 0.625, 1.25, 2.5, 5, 7.5, 10, and 20 µM Incubation Time: 24 hours Result: Inhibited lung cancer cells growth in a concentration-dependent manner. Western Blot AnalysisCell Line: A549 and H460 cells Concentration: 0.625, 1.25, 2.5, 5, 7.5, 10, and 20 µM Incubation Time: 24 hours Result: Decreased p‐Cdc2, Cdc2, and Bcl‐2 expression in 2 lung cancer cells.In VivoL48H37 (intraperitoneal injection; 5 mg or 10 mg/kg; once daily; 11‐day ) inhibits H460 xenograft tumor growth and exhibits anti‐tumor activity in mice . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: 5‐week‐old athymic BALB/cA nu/nu female mice (18‐22 g)Dosage: 5 mg or 10 mg/kg Administration: Intraperitoneal injection; once daily; 11‐day Result: Reduced tumor wet weights as compared to vehicle control. Decreased the levels of p‐STAT3, and increased the levels of p‐EIF2α and ATF4 in vivo. Exhibited no significant structural changes in mice.Form:SolidIC50& Target:TLR4. Specifications and Purity: 97%. Molecular Formula: C27H33NO7. Molecular Weight: 483.55. PubChem CID: 4201646. Isomeric SMILES: CCN1CC(=CC2=CC(=C(C(=C2)OC)OC)OC)C(=O)C(=CC3=CC(=C(C(=C3)OC)OC)OC)C1.