L82-G17 (C09-1142-855)

L82-G17 is an uncompetitive DNA ligase I (Lig I)-selective inhibitor. L82-G17 inhibits the third step of the ligation reaction, phosphodiester bond formation. L82-G17can be used as a probe of the catalytic activity.In VitroL82-G17 (200 μM, 30 min) has selective uncompetitive inhibitory effect for LigI. L82-G17 (0-100 μM) increases LigI binding to non-ligatable nicked DNA binding. L82-G17 inhibits step 3 of the ligation reaction, phosphodiester bond formation. L82-G17 (0-100 μM) inhibits DNA synthesis, cell viability and s induces DNA damage. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: HeLa cells Concentration: 0-30 μM Incubation Time: 5 days Result: Reduced cell number by about 70% at 20 μM. Cell Proliferation AssayCell Line: CH12F3 WT and CH12F3Δ/Δ cells Concentration: 0-100 μM Incubation Time: 72 h Result: Had great effect on the proliferation and survival of the parental CH12F3 cells.Form:Solid. Specification: 0.99 Molecular Formula: C11H9ClN4O2 Molecular Weight: 264.67 PubChem CID: 5711175 Isomeric SMILES: C1=CC(=CC(=C1)O)/C=N/NC2=C(C(=O)NN=C2)Cl