Cell Line Origin
Rat Skeletal muscle myoblast
Cell Line Description
The myogenic cell line L8 was isolated by D Yaffe in 1969 by selective serial passage of myoblasts from primary rat skeletal muscle cell cultures (new born, non-in-bred Wistar rats). Subsequently they were recloned by H Blau. L8 cells differentiate into cross-striated myotubes by fusion and express several muscle-specific proteins. The myoblast population will be depleted if cells are allowed to become confluent before sub-culturing. Therefore, sub-cultivation before confluency and periodical re-cloning is necessary. The preparation of a frozen stock at early passage is recommended.
Application
Study of muscle differentiation, expression and analysis of muscle-specific proteins
Culture Medium
DMEM : Medium 199 (4:1) + 2mM Glutamine + 1% CEE (Chick Embryo extract (Seralab)) + 10% Horse Serum (HS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 cells to 1x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C; sub-cultivate before confluency and reclone periodically.
Other Notes
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biological source: rat muscle. growth mode: Adherent. karyotype: Not specified. morphology: Myoblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41116127
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 95102434-1VL
