Cell Line Origin
Mouse A/He lung adenoma
Cell Line Description
Isolated from a urethan-induced lung adenoma of a 28 week old A/He strain mouse. The cells incorporate high levels of 14C-choline into lecithin. C-type particles have been observed in some cultures.
Application
Tumourigenicity studies
Culture Medium
Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: mouse lung. growth mode: Adherent. karyotype: Unstable, range 38-256 chromosomes. morphology: Epithelial-like. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51161527
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 90040512-1VL
