2D Sample Preparation

A reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry. The protein spots are first excised from the gel and transferred to a proteomic grade tube.

The InGel&trade Blue kit provides a complete set of reagents for the in gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie or fluorescent stained

A 96-well format kit suitable for processing larger numbers of protein spots concurrently and compatible with spot-picking instruments. Fewer than 96 samples a batch may also be processed without any waste.

Add an appropriate volume of the supplied rehydration buffer to the dry, ready-to-use, urea based equilibration buffer. Eliminates worries about urea induced protein carbamylation or waste of unused reagents, as fresh and ready-to-use reagents are

Following in gel digestion of proteins with proteases, the addition of Pep-Extract &trade rapidly elutes digested peptides in under 20 minutes. The eluted peptides can be used directly in mass spectrometry analysis, without any additional clean up.