Heterobifunctional Reagents

A popular non-cleavable, membrane-impermeable, water-soluble DSS analog Category: Amine-reactive Reactive Groups: sulfo-NHS-ester Reactive Toward: amino grops Water Soluble: Yes Membrane Permeable: No Cleavability: Non-cleavable MW: 572.43

A popular non-cleavable, membrane-impermeable, water-soluble DSS analog Category: Amine-reactive Reactive Groups: sulfo-NHS-ester Reactive Toward: amino grops Water Soluble: Yes Membrane Permeable: No Cleavability: Non-cleavable MW: 572.43

Low potential for eliciting an immune response, ensuring that the primary response is to the antigen-carrier protein conjugate and not against a determinant on the crosslinker. Less immunogenic than SMCC EReactive groups: NHS ester and maleimide

Heterobifunctional Reactive groups maleimide and NHS ester Primary amine and sulhydryl reactive Hapten-carrier protein conjugation 1,2 Soluble in DMSO and chloroform Storage: Shipped at ambient temperature, upon receipt store at 4&degC.

Primary amine and sulfhydryl reactive Protein-protein conjugation

NHS LC SPDP, Succinimidyl 6-[3-(2-pyridylthio) propionamido] Hexanoate Spacer arm SPDP Antibody-toxin conjugation

Primary amine and sulfhydryl Protein-protein conjugation

SANH (Succinimidyl 6-hydrazinonicotinamide acetone hydrazone) A heterobifunctional crosslinker used to modify amine-containing biomolecules or surfaces to directly incorporate 2-hydrazinopyridine moieties. This reagent, when combined with SFB is

SFB (Succinimidyl 4-formylbenzoate) A heterobifunctional crosslinker used to modify amine-containing biomolecules or surfaces to directly incorporate benzaldehyde moieties. This reagent, when combined with SANH (Succinimidyl 6-hydrazinonicotinamide

SIAB, N-Succinimidyl (4-iodoacetyl) Aminobenzoate Primary amine and sulfhydryl reactive Enzyme-antibody conjugation

SIAB, N-Succinimidyl (4-iodoacetyl) Aminobenzoate Primary amine and sulfhydryl reactive Enzyme-antibody conjugation

SMCC, Succinimidyl-4-(N-maleimidomethyl) cyclohexane-1-carboxylate Primary amine and sulfhydryl reactive Enzyme-antibody conjugation 1,2