Protein Sample Preparation

The SpinOUT&trade GT-100, GT-600 and GT-1200 columns are versatile, spin-format columns for the desalting and buffer exchange of peptide, protein and nucleic acid solutions ranging from 5&microl to 4ml sample volumes. Also available in 96-well

The SpinOUT&trade GT-100, GT-600 and GT-1200 columns are versatile, spin-format columns for the desalting and buffer exchange of peptide, protein and nucleic acid solutions ranging from 5&microl to 4ml sample volumes. Also available in 96-well

The SpinOUT&trade GT-100, GT-600 and GT-1200 columns are versatile, spin-format columns for the desalting and buffer exchange of peptide, protein and nucleic acid solutions ranging from 5&microl to 4ml sample volumes. Also available in 96-well

The SpinOUT&trade GT-100, GT-600 and GT-1200 columns are versatile, spin-format columns for the desalting and buffer exchange of peptide, protein and nucleic acid solutions ranging from 5&microl to 4ml sample volumes. Also available in 96-well

The SpinOUT&trade GT-100, GT-600 and GT-1200 columns are versatile, spin-format columns for the desalting and buffer exchange of peptide, protein and nucleic acid solutions ranging from 5&microl to 4ml sample volumes. Also available in 96-well

Steptavidin, a bacterial protein isolated from Streptomyces avidinii, is similar to egg-white avidin in its ability to bind biotin, and has been used as a replacement for egg-white avidin because of its more favorable chemical properties. Conjugate:

Streptavidin: Streptavidin is a tetrameric protein composed of four identical subunits. Each subunit binds one biotin molecule with a KD of ~1 x 10-15 M.

Steptavidin, a bacterial protein isolated from Streptomyces avidinii, is similar to egg-white avidin in its ability to bind biotin, and has been used as a replacement for egg-white avidin because of its more favorable chemical properties. Conjugate:

Streptavidin: Streptavidin is a tetrameric protein composed of four identical subunits. Each subunit binds one biotin molecule with a KD of ~1 x 10-15 M.

Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10&sup1&acirc&microM-&sup1) and streptavidin (Ka=10&sup1&acirc&microM-&sup1). Biotin and (strept)avidin interaction is rapid.

Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10&sup1&acirc&microM-&sup1) and streptavidin (Ka=10&sup1&acirc&microM-&sup1). Biotin and (strept)avidin interaction is rapid.

Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10&sup1&acirc&microM-&sup1) and streptavidin (Ka=10&sup1&acirc&microM-&sup1). Biotin and (strept)avidin interaction is rapid.