Lactimidomycin (C09-1142-543)

Lactimidomycin is a glutarimide-containing compound isolated from Streptomyces . Lactimidomycin is a potent inhibitor of eukaryotic translation elongation . Lactimidomycin has a potent antiproliferative effect on tumor cell lines and selectively inhibit protein translation. Lactimidomycin inhibits protein synthesis with an IC 50 value of 37.82 nM. Lactimidomycin is also a potent and non-toxic inhibitor of dengue virus 2 and other RNA viruses. Anticancer and antiviral activitiesIn VitroLactimidomycin (0.01-100 nM; 24 hours; Hs 579T, HCC 1937, HCC 1395, HCC 2218, BT 474, MCF 7, MDA MB231 cells and MCF 10A) treatment inhibits cell growth with IC 50 concentrations in the low nanomolar range, but higher doses are necessary to inhibit growth of the non-tumorigenic breast cell line MCF10A. Lactimidomycin induces a clear dose-responsive inhibition of DENV2 infectious particle production with an EC 90 value of 0.4 μM. No measurable decrease in cell viability was detected at concentrations up to 12.5 µM. Lactimidomycin is a potent inhibitor of DENV2 and Lactimidomycin's inhibition of DENV2 translation leads to reduced production of newly infectious particles. Lactimidomycin may protect cells from viral cytopathic effects including apoptosis, likely through inhibition of virus protein production and replication. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Proliferation AssayCell Line: Hs 579T, HCC 1937, HCC 1395, HCC 2218, BT 474, MCF 7, MDA MB231 cells and MCF 10A Concentration: 0.01-100 nM Incubation Time: 24 hours Result: Inhibited cell growth with IC 50 concentrations in the low nanomolar range.In VivoLactimidomycin (0.6 mg/kg; intraperitoneal injection; daily; for one month; female nude mice) treatment has an appreciable effect on tumor growth in nude mice . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Female nude mice (6-8 week old) with MDA MB 231 cells Dosage: 0.6 mg/kg Administration: Intraperitoneal injection; daily; for one month Result: Had an appreciable effect on tumor growth in vivo.Form:SolidIC50& Target:Eukaryotic translation elongation, Dengue virus 2 and other RNA viruses. Molecular Formula: C26H35NO6 Molecular Weight: 457.56 PubChem CID: 6439383 Isomeric SMILES: CC1/C=C/C=C/CC/C=C/C(=O)OC1/C(=C/C(C)C(=O)CC(CC2CC(=O)NC(=O)C2)O)/C