LB-Agar is a rich Medium for the growth of E. coli and certified for plasmid and other bacterial cultures in liquid medium. Molecular Biology Certified Widely used in molecular biology Nutritionally rich medium Supports a luxurious growth of E. coli cells Tryptone and yeast extract supply essential growth factors Contains essential electrolytes for transport and osmotic balance The two main components of LB media are Tryptone and Yeast Extract. Tryptone is used in a concentration of 10 gram / liter and Yeast Extract in a concentration of 5 gram / liter. LB medium (Miller) Agar is the high salt LB medium type with 10 gram /liter and 15 gram/liter Agar. Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in LB Agar Medium formulations. Chosen reagents are combined in the proper proportions according to the LB Agar formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation is used to prepare agar medium. The combinations are mixed to dissolve components and then autoclaved at 121°C for 15 minutes.
- UPC:
- 41111515
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 113002232
