LECTIN FROM LYCOPERSICON ESCULENTUM (TOMATO) FICT CONJUGATE

General description

Lectins are carbohydrate binding proteins. Lectin from Lycopersicon esculentum (LEA) binds strongly to polylactosamine oligosaccharides. It is the major component of endothelial and epithelial cell surface carbohydrate.

Application

Lectin from Lycopersicon esculentum (tomato) has been used:

• for immunofluorescence staining
• for the quantification of vascular perfusion
• for the evaluation of gel perfusion efficiency

Biochem/physiol Actions

Lectin has anti-cancer, anti-human immunodeficiency virus (anti-HIV), anti-microbial infection and blocks mucosal atrophy. It reduces type 2 diabetes and obesity, stimulates nutrient absorption and drug targeting. It is used to stain the vascular tree in the spinal cord and brain of animals. Lectin agglutinates cells and precipitates polysaccharides and glycoproteins.

LEA is not blood group specific, but has an affinity for N-acetyl-β-D-glucosamine oligomers. The lectin is a glycoprotein containing approx. equal amounts of protein and carbohydrate and is reported to inhibit the mitogenic activity of phytohemagglutinin from Phaseolus vulgaris. This lectin binds endothelial cells and is used to visualize the microvasculature.

Packaging

Sold on the basis of mg active conjugate.

Physical form

Solution in 10 mM HEPES, 0.15 M NaCl, pH 7.5, containing 0.1 mM Ca²⁺, 0.08% sodium azide and 5 mg/mL β-cyclodextrin.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.3, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25 °C.