Detects glycoproteins containing β(1→4)-N-acetyl-D-glucosamine when used with appropriate peroxidase substrateGeneral Western Blot Protocol:Glycoprotein sample size: 500ngLectin Concentration: 0.1ug/mlLoad samples at 500 ng of glycoprotein per laneRun 4-20% Bis-Tris SDS page gelTransfer gel to a PVDF membraneBlock membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RTWash membrane 5 x 5 minutes with 25ml RIPA bufferDetect using chemiluminescent substrate (CPS1-120)
Specification: peroxidase conjugate, lyophilized powder
- UPC:
- 41181736
- Condition:
- New
- Availability:
- 4-8 weeks
- Weight:
- 0.07 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- L755095-2mg
- Product Size:
- 2mg
