Skip to main content

Leptospira Medium Base, 500 g

Catalog No.
C003B-007623
Mfr. No.
M1009-500G
Mfr. Name
HIMEDIA LABORATORIES, LLC
Qty/UOM
1
UOM
EA
Price: $323.79
List Price: $359.77

Leptospirosis is an acute febrile disease caused by members of the genus Leptospira (1,2). Direct culture of blood is the most reliable way to detect Leptospira during the first week of illness.

Enjoy exclusive benefits including discounted pricing on orders by contacting our Sales Executives to open an account. Contact Us

Adding to cart… The item has been added
Leptospirosis is an acute febrile disease caused by members of the genus Leptospira (1,2). Direct culture of blood is the most reliable way to detect Leptospira during the first week of illness. After the first week of illness and for several months thereafter, leptospires may be isolated by direct culture of undiluted urine specimens. By autopsy, leptospires may be isolated from kidney and liver tissues as well as from blood and urine. The Leptospira Medium Base was originally formulated by Ellinghousen and McCullough (3) and modified by Johnson and Harris (4). Leptospira Medium Base is enriched by the addition of Leptospira Enrichment. Leptospira Enrichment supplement provides long chain fatty acids as the carbon, energy source and vitamin for the growth of Leptospira . The salts supply essential nutrients for the growth of the organisms. Phosphates form buffering system while sodium chloride maintains osmotic equilibrium and also provides essential ions. Leptospira metabolizes the fatty acids by beta-oxidation and the metabolic end products formed are acetate and carbon dioxide. All cultures are incubated at room temperature in the dark for up to 6 weeks. The organisms grow below the surface. Material collected from a few centimeters below the surface of broth cultures should be examined weekly for the presence of growth using a direct wet preparation under dark field illumination. Letpospires will exhibit corkscrew like motility (1). Examine the tubes for growth every 5-7 days. Growth occurs as a ringed area (disc) 1-3 cm below the surface of the medium. The absence of a ringed area of growth doesnt necessarily mean leptospires are not present. Remove a small amount of growth from the disc area and examine microscopically (gram stain is not satisfactory). Microcolonies can be fixed with methanol and stained with Giemsas stain to show rod forms (5). Storage and Shelf-life: Store below 30°C in tightly closed container and prepared medium at 2-8°C. Use before expiry period on the label. References: 1.Forbes B. A., Sahm A. S., and Weissfeld D. F., Bailey & Scotts Diagnostic Microbiology, 10th Ed., 1998, Mosby, Inc., St. Louis, Mo. 2.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology, 8th Ed., American Society for Microbiology, Washington, D.C. 3.Ellinghausen and McCullough, 1965, Am. J. Vet. Res., 26:39. 4.Johnson and Harris, 1967, J. Bact., 94:27. 5.Korthof G., 1932, Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. I. Orig., 125:429.
UPC:
41202036
Condition:
New
Availability:
3 Days
Weight:
1.00 Ounces
HazmatClass:
No
WeightUOM:
LB
MPN:
M1009-500G
Model Number:
C979Z67

Cenmed Satisfaction Guarantee
Cenmed Satisfaction Guarantee

At Cenmed, your confidence and satisfaction are paramount. We guarantee the quality and reliability of our extensive range of clinical and laboratory supplies. If you're not completely satisfied with your purchase, we offer a straightforward return process and dedicated support to resolve your concerns promptly. Our commitment ensures that you can order with confidence, knowing that Cenmed is dedicated to superior service and customer satisfaction. Trust us to meet your needs with every order, backed by our promise of excellence. Learn more in Help & FAQs.

Testimonial
"Cenmed provides me access to the same products/services normally reserved for much larger labs than mine. I was presently surprised by their product offering."

LAB DIRECTOR

Testimonial
"We utilized Cenmed's capabilities for a variety of projects around the world. They are a valued partner and supplier."

PHARMACEUTICAL SUPPLY CHAIN LEADER

Testimonial
"The reps are very good at finding products for customers in this period of supply chain issues."

SCOTT BEHMAN

Testimonial
"Your customer service has been excellent and makes me excited about purchasing with Cenmed in the future!!"

PROCUREMENT + BILLING COORDINATOR AT PHARMA.