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LIPOPOLYSACCHARIDES FROM ESCHERICHIA COLI K-235 PURIFIED BY GEL FILTRATION CHROMATOGRAPHY (C15-1470-575)

Sigma Aldrich

Catalog No.
C15-1470-575
Manufacturer No.
L2018-5MG
Manufacturer Name
Sigma-Aldrich
Quantity
5
Unit of Measure
MG
Price: $433.50
List Price: $481.66

This product is extracted from E. coli serotype K235 and purified by gel filtration.

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General description

This product is extracted from E. coli serotype K235 and purified by gel filtration. The source strain is ATCC 13027.

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

UPC:
12352200
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
WeightUOM:
LB
MPN:
L2018-5MG

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