Liqui-Gel is a 40% (w/v) solution of Ultra Pure Acrylamide (38.96%) and bis-Acrylamide (1.04%) in specially-prepared deionized water. Final ratio is 37.5:1. Acrylamide decomposes in the presence of acids, bases, oxidizing agents, iron, and iron salts. It decomposes non-thermally to form ammonia, and thermal decomposition produces carbon monoxide, carbon dioxide, and oxides of nitrogen. Polyacrylamide gel electrophoresis is a versatile method of separation, characterization, and analysis of nucleic acids, proteins and other charged materials. The gels are formed by the polymerization of the acrylamide monomer with a co-monomer, often N,N'-Methylene-bis-acrylamide, which acts as a cross-linking agent, in the presence of free radicals or a photochemical initiator.It has many other uses in molecular biology laboratories, including the use of linear polyacrylamide (LPA) as a carrier, which aids in the precipitation of small amounts of DNA. Liqui-Gel™ 37.5:1 was used for Denaturing gradient gel electrophoresis (DGGE) analyses following PCR amplification step of a 500 bp region of the 16S-rRNA gene.
- UPC:
- 41115700
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazMatClass:
- Yes
- WeightUOM:
- LB
- MPN:
- 4800804
