Litmus Lactose Agar, 5KG

Numerous plating media are in use today for the differentiation of lactose-fermenters and lactose non-fermenters. Some of these are selective, whereas others are differential. Some lactose fermenting, gram-negative enteric bacteria can tolerate the inhibitory substances present in the media. These bacteria can be recognized readily by their appearance on selective plates. Litmus Lactose Agar is formulated by Wurtz for the differentiation of lactose fermenting and lactose non-fermenting bacteria. Meat peptone and beef extract in the medium provide nitrogenous nutrients to the organisms. Lactose is fermented by lactose fermenting bacteria with acid production. Litmus is the pH indicator, which turns red at acidic pH. Colonies of lactose fermenting bacteria are surrounded by a red zone, which distinguishes them from colonies of other organisms that either do not change the surrounding medium or change it to blue due to production of ammonia. Inoculate culture from primary fermentation tubes showing gas either by streaking directly or by pour plate method of serially diluted culture. Storage and Shelf-life: Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.