Liver Meat Agar, 500 g

Anaerobic bacteria live in an oxygen-free environment. Some anaerobic bacteria actually die if oxygen is present, while others fail to grow and multiply (1). Meat liver base provides adequate degree of anaerobiosis and is also rich source of growth nutrients, which enables even the strict and fastidious anaerobes to grow well. Some anaerobes (e.g. certain Clostridium species) reduce the sulphite present in the medium to hydrogen sulphide (H2S) which is indicated by the blackening of colonies due to presence of ferric ammonium citrate. Inoculation can be performed by the pour plate method or by surface smearing. Storage and Shelf-life: Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. Reference: 1.Alcamo E. I., 2001, Fundamentals of Microbiology, 6th Ed., Jones and Bartlett Publishers