Cell Line Origin
Human diploid lung
Cell Line Description
Derived from normal tissue taken at post-mortem from the lung of a 5 year old male. An ampoule of cells frozen after one passage and estimated population doubling (PDL) 2 was submitted to the American Type Culture Collection in June, 1977. Derived populations at passage 6 and approximately 14 PDL are available for distribution. Progeny were found to undergo an additional 25 population doublings in longevity trials.
Application
Tumourigenicity studies
Culture Medium
Ham′s F12 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Finite lifespan, a further 20 population doublings expected.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
biological source: human lung. growth mode: Adherent. karyotype: 2n : 46. diploid. morphology: Fibroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41181936
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 90073001-1VL
