Cell Line Origin
Human Caucasian diploid lung
Cell Line Description
Derived from normal tissue of an 18 year old Caucasian male with osteogenic sarcoma.
Application
Tumourigenicity studies
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 12
D16S539: 9,13
D5S818: 12,13
D7S820: 11
THO1: 6,7
TPOX: 8
vWA: 16,18
Culture Medium
Coon′s modified Ham′s F12 + Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. A further 10 population doublings expected.
Other Notes
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biological source: human lung. growth mode: Adherent. karyotype: 2n : 46. diploid. morphology: Fibroblast-like. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41105328
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 90102541-1VL
