Cell Line Origin

Human Caucasian prostate carcinoma

Cell Line Description

Derived from a metastasis at the left supraclavicular lymph node of a 50 year old patient with a confirmed diagnosis of metastatic prostate carcinoma. Growth and acid phosphatase production is affected by 5-alpha-dihydrotestosterone. They do not form a uniform monolayer and attach only lightly to the substrate. When shipped, cells detach from flask and can either be incubated 24-48 hours to allow attachment or be collected by centrifugation (150xg, 15 minutes) and reseeded.

Application

LNCap Clone FGC Cell Line has been used:

to study the effect of sex steroid hormones in regulating the expression of oligoadenylate synthetase 1 (OAS1)
to study the role of curcumin and resveratrol in redox control and ability to promote apoptosis
to investigate low molecular weight thiols present in the deproteinized extract of the cell line after derivatization

Culture Medium

RPMI 1640 + 2mM Glutamine + 1.0 mM sodium pyruvate + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37Â°C.i.e. seeding at 1-3 x 10,000 cells/cm² Cells grow slowly in clusters and can be disaggregated by repeated pipetting. After subculture cells may take up to 48 hours to reattach. Do not disturb during this period. Medium is rapidly acidified, therefore change twice per week.

Other Notes

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biological source: human prostate (carcinoma). growth mode: Adherent. karyotype: Pseudodiploid male. seven marker chromosomes, modal number 46, range 33 to 91. morphology: Epithelial-like. products: Prostate acid phosphatase, prostate specific antigen. receptors: Androgen, estrogen. technique(s): cell culture | mammalian: suitable. relevant disease(s): metastasis. shipped in: dry ice. storage temp.: −. 196Â°C.