Cell Line Origin
Rat Wistar spleen x Mouse P3X63Ag8.653
Cell Line Description
LR-3 hybridoma cells were produced by immunisation of Wistar rats with bone marrow from Mesocestoides cortii infected BALB/c cells. Subsequently rat spleen cells were fused with P3X63Ag8.653 myeloma cells.
Immunogen
Bone marrow from Mesocestoides cortii infected BALB/c mice
Application
Detection of murine B cells, mAb appear to stimulate B cell proliferation. Detection of HSA by immunopricipitation.
The isotype of the monoclonal antibody produced by this cell line is rat IgM.
Culture Medium
DMEM + 2mM glutamine + 5-10% Foetal Bovine Serum (FBS), feeder layer improves growth
Subculture Routine
Maintain cultures between 1-2x100,000 cells/ml; 5% CO2; 37°C
Other Notes
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biological source: Rat x Mouse Hybridoma. ImmunoDon: Rat Wistar spleen cells. Myeloma: P3X63Ag8.653. Reactivity: Anti-Heat Stable Antigen, mouse. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41116127
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 93062221-1VL
