Luria Agar is prepared as described by Lennox (1) for cultivation and maintenance of recombinant strains of Escherichia coli . The media is generally used for molecular and genetic studies, because of its nutritive capacity and simple composition, which can be easily altered as per specific requirements. The medium is nutritionally rich for the growth of pure cultures of recombinant strains. Strains which are generally derived from Escherichia coli K12 are deficient in Vitamin B synthesis and are further modified by specific mutation to create auxotrophic strains that are unable to grow on nutritionally deficient media. Casein enzymic hydrolysate provides peptides and peptones while Vitamin B complex is provided by yeast extract. Sodium chloride provides sodium ions for the membrane transport and maintains osmotic equilibrium of the medium. Directions: Suspend 35.0 grams in 1000 ml distilled water. Heat to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Dispense as desired.
- UPC:
- 41116111
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- M557-500G
- Model Number:
- C992D31
