Lysine Iron Agar, 2.5KG

Lysine Iron agar is recommended for differentiation of enteric organisms especially Salmonella arizonae, based on their ability to decarboxylate or deaminate lysine and to form hydrogen sulphide (H2S). Dextrose is a source of fermentable carbohydrate. Ferric ammonium citrate and sodium thiosulphate are indicators of H2S formation. Cultures that produce hydrogen sulphide cause blackening of the medium due to ferrous sulphide production. Lysine decarboxylation causes an alkaline reaction (purple color) to give the amine cadaverine and the organisms which do not decarboxylate lysine, produce acid butt (yellow color). Application: For Laboratory Chemicals, Analytical Purpose, Biochemical Analysis Appearance: Light yellow to greyish yellow homogeneous free flowing powder Gelling: Firm, comparable with 1.5% Agar gel Color and Clarity of prepared medium: Purple colored, clear to slightly opalescent gel forms in tubes as slants Reaction: Reaction of 3.45% w/v aqueous solution at 25°C. pH : 6.7±0.2 Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours