Lysine Lactose HiVeg Broth, 5KG

Lysine Lactose HiVeg™ broth is used for determination of lysine decarboxylase activity of lactose non-fermenting members of Enterobacteriaceae, especially Salmonellae. Dextrose and lactose are the fermentable sugars. L-Lysine is the substrate that is decarboxylated due to decarboxylase enzyme activity. Bromocresol purple acts as the pH indicator. The enteric bacilli produce acid in an initial fermentation (lactose). Lactose non-fermenters produce acid from dextrose resulting in the formation of yellow color. Subsequently L-Lysine is decarboxylated to form cadaverine resulting in an alkaline reaction and the broth reverts to purple color. Lysine Lactose HiVeg™ broth is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks. Application: For Laboratory Chemicals, Analytical Purpose, Biochemical Analysis Appearance of powder: Light yellow colored, may have slightly greenish tinge, homogeneous, free flowing powder Color and Clarity: Purple colored, clear solution without any precipitate Reaction: Reaction of 2.4% w/v aqueous solution is pH 6.8 ± 0.2 at 25°C Cultural Response: Cultural characteristics observed after an incubation at 35-37°C after 24 hours