General description
Lysozyme is abundantly found in animal and plant kingdoms. It is a natural food preservative and also found to be present in specific bacterial cell walls. Lysozyme is usually present in tears, milk, urine and saliva.
Application
Lysozyme from chicken egg white has been used:
- in dielectric spectroscopy studies of dynamics of protein
- as a control to measure the human lysozyme activity
- as a supplement in soaking solution to treat lenses
Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
Biochem/physiol Actions
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
Lysozymes participate in the defense mechanism. It has the ability to stimulate catalysis by bringing steric stress in the substrates.
Features and Benefits
- Highly purified by repeated crystallization and dialysis
- Each lot is use-tested for isolation of plasmid DNA from E. coli
Unit Definition
One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25°C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).
Preparation Note
3× crystallized
- UPC:
- 51284030
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- L4919-5G
- CAS:
- 12650-88-3