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LYSOZYME CHICKEN EGG WHITE FOR USE AS A MARKER IN SDS-PAGE (C005B-459340)

Catalog No.
C005B-459340
Mfr. No.
L4631-1VL
Mfr. Name
Sigma-Aldrich
Qty/UOM
1
UOM
VL
Price: $288.66
List Price: $320.73

Application Enzyme breaks down the cell walls of bacteria: used to prepare spheroplasts. Biochem/physiol Actions Lysozyme hydrolyzes β:(1→:4) linkages between N -acetylmuramic acid and N -acetyl- D -glucosamine residues in

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Application

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Biochem/physiol Actions

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

UPC:
12352204
Condition:
New
Availability:
3-5 Days
Weight:
1.00 Ounces
HazmatClass:
No
MPN:
L4631-1VL
CAS:
12650-88-3

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