Lyticase from Arthrobacter luteus (C15-1235-577)

Application

Lyticase from Arthrobacter luteus has been used to lyse the fungal cell wall for DNA isolation.

Biochem/physiol Actions

Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase to induce partial spheroplast formation. Spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest the cell walls of yeast and generate spheroplasts from fungi for transformation. It contains β-(1→3)-glucan laminaripentaohydrolase along with β-(1→3)-glucanase, protease, and mannanase activities. Lyticase is used for yeast cells like Candida, Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species.

Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.

Unit Definition

One unit will produce a ΔA₈₀₀ of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.

Physical form

Partially purified, lyophilized powder containing potassium phosphate buffer salts and stabilizers

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer