M-BCG Yeast and Mold Broth, 500G

M-BCG (Bromo Cresol Green) Yeast and Mould Broth is used for detecting fungi in routine analysis of beverages using membrane filter technique. It is a modification of M-Yeast and Mold Broth used for detection of fungi in sugar and other materials. The medium is highly nutritious for the growth of yeasts and moulds. Biopeptone and yeast extract provide nitrogenous compounds and vitamin B complex. Thiamine is also a B vitamin in the medium. Dextrose acts as the energy source. Diastase is a mixture of amylolytic enzymes. Bromo cresol green is the pH indicator which is green at acidic pH (pH 4.0) while blue at pH 5.6. Potassium phosphate helps in maintaining buffering action in the medium. The low pH inhibits bacterial growth. The membrane filter pad is saturated with 2.0 to 2.5 ml broth. Place the membrane filter used for filtration of test sample on the saturated pad and incubate at 30 -35°C for 48 hours. Storage and Shelf-life: Store below 30°C in tightly closed container and use freshly prepared medium. Use before expiry date on the label.