M-GREEN YEAST AND MOLD BROTH

General description

m-Green Yeast and Mold Broth is a relatively more complex formula compared to other media used for the isolation of fungi and yeast. This formulation is rich in nutrients which allows excellent fungal growth. Bacterial growth is inhibited by an acidic pH. Enzymatic digest of casein and enzymatic digest of animal tissue provide nitrogen, carbon, and amino acids in m-Green yeast and mold Broth. Yeast extract is the vitamin source. Dextrose is an energy source for metabolism of fungi. Potassium phosphate is a buffering agent. Magnesium sulphate, Thiamine, and Diastase (a mixture containing amylolytic (starch) enzymes) provide essential ions, minerals, and nutrients. Bromocresol Green is the pH indicator, facilitating visualization and counting of fungal colonies. The colonies are green due to the diffusion of bromocresol green into the colonies (alkaline reaction). Acidic end products from the colonies diffuse into the medium, further reducing the pH and causing the dye to turn yellow (acid reaction). Yeast produces green, opaque large colonies. Mold colonies are green and filamentous.

Application

m-Green yeast and mold Broth is used for the testing soft drinks and other beverages like beer, bottled water, cider, soft drink, sport drink and wine for growth of yeast and mold.

Features and Benefits

• Ready-to-use Liquid ampoule media solve numerous problems in microbiology laboratories where time, equipment, and trained personnel are often in short supply.
• They are available in convenient, single-test 2 mL plastic ampoules, eliminating the manipulation of glass ampoules and avoiding the waste and spoilage associated with bulk packaged media.

Specifications

Incubation Temperature: 28–32 °C

Incubation Time: 48–72 hrs

Analysis Note

QC organisms
S. cerevisiae (ATCC 9763)

A.brasilliensisformerly A. niger (ATCC 16404 / WDCM 00053)

Target Microorganisms: Yeast and mold (selection by pH)

Yeast produce green, opaque large colonies. Mold colonies are green and filamentous

Legal Information

EZ-FIT is a registered trademark of Merck KGaA, Darmstadt, Germany

MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany