General description
Moloney murine leukemia virus (M-MLV ) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues. It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.
Application
M-MLV Reverse Transcriptase has been used:
- for the preparation of cDNA libraries or for first strand cDNA synthesis
- for use in a 2-step RT-PCR assay
- in quantitative realtime-polymerase chain reaction (RT-qPCR)
- in reverse transcription
Features and Benefits
- Thermostable reverse transcriptase active at 37 °C.
- Can be used to generate first strand cDNA of up to 7 kb.
Packaging
Supplied with 10× M-MLV reverse transcriptase buffer containing DTT.
Unit Definition
One unit incorporates 1 nmol of TTP into acid precipitable material in 10 min. at 37 °C using poly(A):oligo dT as a template:primer.
Preparation Note
The enzyme is purified from Escherichia coli expressing the pol gene of M-MLV on a plasmid.
Legal Information
Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
- UPC:
- 12352204
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- M1302-40KU
- Product Size:
- 40000/UNITS
- CAS:
- 9068-38-6
