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MacConkey Agar without CV with 0.15% Bile Salt. Agar used for the selective isolation and differentiation of lactose fermenting and lactose nonfermenting enteric bacteria. Pack size is 500G

Thomas Scientific

Catalog No.
C08-0442-977
Manufacturer No.
M008A-500G
Manufacturer Name
HIMEDIA LABORATORIES, LLC
Quantity
1
Unit of Measure
EA
Price: $252.88
List Price: $280.98

MacConkey Agar Medium is the earliest selective and differential medium for cultivation of enteric microorganisms from a variety of clinical specimens (2, 3). Subsequently MacConkey Agar is recommended for use in microbiological examination of

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MacConkey Agar Medium is the earliest selective and differential medium for cultivation of enteric microorganisms from a variety of clinical specimens (2, 3). Subsequently MacConkey Agar is recommended for use in microbiological examination of foodstuffs (4) and for direct plating / inoculation of water samples for coliform counts (5). This medium is also accepted by the Standard Methods for the Examination of Milk and Dairy Products (6) and pharmaceutical preparations (1). The original MacConkey Agar incorporated peptones, lactose bile salts and two dyes. MacConkey Agar w/o CV w/ 0.5% Bile salts is a modification of the original medium with the exception of crystal violet. Peptic digest of animal tissue serves as the source of essential nutrients. Lactose is the fermentable carbohydrate with neutral red serving as the pH indicator. Sodium chloride maintains the osmotic equilibrium of the medium. Bile salts serve to make the medium selective. Lactose fermenting strains grow as red or pink and may be surrounded by a zone of acid precipitated bile. The red colour is due to production of acid from lactose, absorption of neutral red and a subsequent colour change of the dye when the pH of medium falls below 6.8. Lactose non-fermenting strains, such as Shigella and Salmonella are colourless and transparent and typically do not alter appearance of the medium. Directions: Suspend 52 grams in 1000 ml distilled water. Heat to boiling with gentle swirling to dissolve the agar completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Avoid overheating. Cool to 45-50°C and pour into sterile Petri plates. The surface of the medium should be dry when inoculated.
UPC:
41106200
Condition:
New
Availability:
3 Days
Weight:
1.00 Ounces
HazmatClass:
No
WeightUOM:
LB
MPN:
M008A-500G


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