MacConkey Broth Purple with BCP. Broth used for presumtive i (C08-0442-853)

MacConkey Broth Purple w/ BCP is a modification of MacConkey Medium (1). Childs and Allen (2) demonstrated the inhibitory effect of neutral red and therefore substituted it by the less inhibitory bromocresol purple dye. BCP is more sensitive in recording pH variation in the medium. Peptic digest of animal tissue provides essential growth nutrients. Lactose is the fermentable carbohydrate. Sodium taurocholate inhibits gram-positive organisms. Sodium chloride maintains the osmotic balance of the medium. Bromocresol purple is the pH indicator in the medium which turns yellow under acidic condition. Lactose fermention turn the medium yellow due to the acidity produced on lactose fermentation. The colour change of the dye is observed when the pH of the medium falls below 6.8. Lactose non-fermenting organisms like Salmonella and Shigella do not alter the appearance of the medium. Liquid specimens are directly inoculated while solids have to be homogenized in appropriate diluents such as physiological saline, phosphate buffers, etc. The inoculation must be effected at 10% v/v in Durhams tubes. If the inoculum is greater than 1 ml, it is necessary to use the medium at double strength, inoculating equal volumes of specimen and medium. Directions: Suspend 40.01 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium and distribute into test tubes with inverted Durham tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.