General description
Malate dehydrogenase (MDH) is an enzyme which reversibly catalyzes the oxidation of L-malate to oxaloacetate in the presence of NAD. There are 2 isoforms in eukaryotic cells: MDH1 and MDH2. MDH1 is found in the cytoplasm and plays a key part in the malateaspartate shuttle for transporting malate into the mitochondria. MDH2 is a mitochondrial enzyme which participates in the TCA cycle that reversibly converts L-malate into oxaloacetate. Higher MDH activities are found in some neurodegenerative diseases such as Alzheimer′s disease.
Features and Benefits
- Linear detection range: 0.5 to 65 U/L
- Compatible with high-throughput handling systems.
Suitability
The kit is suitable for malate dehydrogenase activity determination in biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media.)
Principle
The Malate Dehydrogenase Assay Kit is a non-radioactive, colorimetric MDH assay based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
- UPC:
- 41141625
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- MAK512-1KT
- Product Size:
- 1/EA
