Solution I - Aniline Blue
Solution II - 0.5% Acid Fuchsin

Demonstrates collagenous fibrils, cartilage, bone, amyloid, and nuclei.

CONTENTS: Aniline Blue, Orange G, Phosphotungstic Acid, Acid Fuchsin

These solutions are made from certified dyes.

FOR IN VITRO DIAGNOSTIC USE ONLY.

RECOMMENDED PROCEDURE: Sections should be Zenker-fixed and procedure for removal of pigments and precipitates should be followed prior to staining. Stain with Sol. II for 1 - 5 minutes or longer. (Omit Sol. II to highlight collagenous fibrils). Transfer directly to Sol. I without washing in water. Stain for 20 - 60 minutes or longer. Dehydrate, clear and mount. For celloidin sections, shorten staining time, decolorize, dehydrate in 95% ethanol and clear by the blotting paper xylol method or in terpineol and mount in balsam.

RESULTS: Ground substances of cartilage and bone, mucus, amyloid - varying shades of blue; nuclei, fibroglia, myoglia and neuroglia fibrils, axis cylinders and fibrin -red; erythrocytes and myelin - yellow; elastic fibrils - pale pink, pale yellow or unstained.

Safety Data Sheet - Mallory's Aniline Blue Collagen Stain Kit (Solution I)

Safety Data Sheet - Mallory's Aniline Blue Collagen Stain Kit (Solution II)