Manzamine A hydrochloride (C09-1153-057)

Manzamine A hydrochloride, an orally active beta-carboline alkaloid, inhibits specifically GSK-3β and CDK-5 with IC 50 s of 10.2 μM and 1.5 μM, respectively. Manzamine A hydrochloride targets vacuolar ATPases and inhibits autophagy in pancreatic cancer cells. Manzamine A hydrochloride has antimalarial and anticancer activities. Manzamine A hydrochloride also shows potent activity against HSV-1.In VitroManzamine A (5-50 µM, 18 h) hydrochloride decreases tau phosphorylation, measured with ELISA. Manzamine A (10 µM) hydrochloride inhibits yeast S. cerevisiae growth by 30%. Manzamine A hydrochloride displays a few enlarged vacuoles in yeast. Manzamine A (2.5-10 µM, 24 h) hydrochloride increases acidity in pancreatic cancer cells and non-malignant Vero cells. Manzamine A (1 µM, 24 h) hydrochloride inhibits HSV-1 infection in SIRC cells . Manzamine A hydrochloride shows antimalarial activity with an IC 50 of 8.0 nM (D6 clone) and 11 nM (W2 clone) . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability Assay Cell Line: SIRC cell Concentration: 0.1, 0.5, 1, 2, 3, 5, and 10 µM Incubation Time: 72 h Result: Inhibited SIRC cell viability with an IC 50 of 5.6 µM.In VivoManzamine A (50 and 100 mol/kg, p.o. or i.p.) hydrochloride inhibits the growth of the rodent malaria parasite Plasmodium berghei in infected mice . Manzamine A (8 mg/kg, i.p., daily for 8 consecutive days) hydrochloride prolongs the survival of SW mice to 20 days. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Plasmodium berghei in infected mice Dosage: 50 or 100 mol/kg Administration: Intraperitoneal injection (i.p.) or oral administration (p.o.) Result: Inhibited the growth of the rodent malaria parasite Plasmodium berghei .Prolonged the survival of highly parasitaemic mice.Form:Solid. Specifications and Purity: 99%. Molecular Formula: C36H45ClN4O. Molecular Weight: 585.22. PubChem CID: 49797386. Isomeric SMILES: C1CCN2CC[C@H]3C(=C[C@@](CC/C=C\C1)([C@H]4[C@]3(C2)C[C@H]/5N4CCCC/C=C5)O)C6=NC=CC7=C6NC8=CC=CC=C78.Cl.