MAP Kinase 1, His.Tag, Human, Recombinant, E. coli (C15-1303-694)

General description

Recombinant, human MAP Kinase 1 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of purified, recombinant MAP kinase in vitro with MEK1. Suitable for labeling MAP kinase substrates or for inhibitor screening.

Recombinant, human MAP Kinase 1 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP Kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C.

Physical form

In 100 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 50% glycerol, pH 8.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Charest, D.L., et al. 1993. Mol. Cell Biol. 13, 4679.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

recombinant: expressed in E. coli. Quality Level: 100. Assay: ≥. 95% (SDS-PAGE). form: liquid. specific activity: ≥. 6000 . units/mg protein. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. foreign activity: Proteases, none detected. shipped in: wet ice. storage temp.: −. 70°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.