MCF7/S0.5

General description

Oestrogen receptor and progesterone receptors.

Cell Line Description

This MCF7 subline was established by stepwise reductions in the serum concentration from 5% fetal calf serum to 0.5 % in growth medium consisting of DMEM: Ham′s F-12 medium (1:1) supplemented with 2mM glutamine and insulin (6ng/ml). MCF7/S0.5 expresses both oestrogen receptor and progesterone receptors.

Culture Medium

Phenol red free DMEM:F12 (1:1) (D6434) + 1% FBS (F2442), 2.5 mM Ala-Gln (G8541) + 6 ng/ml insulin.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10, 000 cells/cm² using 0.25% trypsin or trypsin or 0.05% trypsin/EDTA solution; 5% CO₂; 37°C.
Recommend media changes every 2-3 days

Other Notes

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Citation

If use of this culture results in a scientific publication, it should be cited in the publication as: MCF7/S0.5 (ECACC 16022501)

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.